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Home >> Antibodies >> HSPB1 Antibody / HSP70

HSPB1 Antibody / HSP70 [clone rHSPB1/7298] (V5206)

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  Catalog No Formulation Size Price (USD)  
Image V5206-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V5206-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V5206SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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IHC staining of FFPE human renal cell carcinoma tissue with HSP27 antibody (clone rHSPB1/7298). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human renal cell carcinoma tissue with HSP27 antibody (clone rHSPB1/7298). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rHSPB1/7298
Purity Protein A/G affinity
UniProt P04792
Localization Cytoplasm, Nucleus
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This HSPB1 antibody is available for research use only.
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Description

HSP27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins produced by all organisms studied. HSP27 synthesis is induced by elevated temperature, as well as by estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSPs and mammalian Alpha-crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. Therefore, HSP27 may be useful in classifying various hormone sensitive tumors.

Application Notes

Optimal dilution of the HSPB1 antibody should be determined by the researcher.

Immunogen

Recombinant human full-length protein was used as the immunogen for the HSPB1 antibody.

Storage

Aliquot the HSPB1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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